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Bachem
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Immudex
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Proimmune
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Abnova
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EZBiolab Inc
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Genemed Synthesis
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Genemed Synthesis
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GL Biochem
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Merck KGaA
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Image Search Results
Journal: PLoS ONE
Article Title: Regulatory B Cells Inhibit Cytotoxic T Lymphocyte (CTL) Activity and Elimination of Infected CD4 T Cells after In Vitro Reactivation of HIV Latent Reservoirs
doi: 10.1371/journal.pone.0092934
Figure Lengend Snippet: ( a ) 500 nM SAHA-treated total or Breg-depleted PBMC from HIV EC (n = 4) and HIV ART (n = 6) were cultured for 4 days and the frequency CD107a + CD8 + T cells determined by flow cytometry. ( b ) After 4 days in culture, by flow-cytometry using an HLA-A*0201 MHC-I HIV Dextramer® (Immudex), the frequency of HIV gag SL9 + CD8 + T cells was determined; left panel depicts representative dot-plots demonstrating specific binding and right panel shows the summary of results (A2 pos = HLA-A*2 positive, A2 neg = HLA-A*2 negative). p values for differences as calculated by paired one-tailed t test (Graphpad Prism software) are indicated.
Article Snippet: The frequency of antigen-specific CD8 + T cells was determined by binding to APC-labeled
Techniques: Cell Culture, Flow Cytometry, Binding Assay, One-tailed Test, Software